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Image Search Results
Journal:
Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization
doi: 10.1128/AEM.68.8.4081-4089.2002
Figure Lengend Snippet: In situ identification of C. pneumoniae in HeLa 229 cells by FISH using Cy5-labeled probe Cpn-974, specific for C. pneumoniae (A) (showing an infra-red fluorescence, assigned to blue color); FLUOS-labeled probe Chlae-574, which targets all members of the family Chlamydiaceae (B) (green); and Cy3-labeled probe Chls-523, which hybridizes to all chlamydiae and chlamydia-like bacteria (C) (red). (D) Due to the overlap of colors, the chlamydial inclusions, which bound all three probes, appear white in the composite image. Bar, 10 μm.
Article Snippet: Chlamydial strains were maintained on cycloheximide-treated
Techniques: In Situ, Labeling, Fluorescence
Journal:
Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization
doi: 10.1128/AEM.68.8.4081-4089.2002
Figure Lengend Snippet: Coinfection of HeLa 229 cells with C. trachomatis and C. pneumoniae. The simultaneous application of FLUOS-labeled probe Ct-623 specific for C. trachomatis (A) (green) and Cy5-labeled probe Cpn-214 specific for C. pneumoniae (B) (blue) allows the differentiation between the two chlamydia species. The simultaneous use of the Cy3-labeled eukaryotic probe EUK516 (C) (red) clearly demonstrated that a single HeLa 229 cell can be infected with both chlamydiae (D). Bar, 10 μm.
Article Snippet: Chlamydial strains were maintained on cycloheximide-treated
Techniques: Labeling, Infection
Journal:
Article Title: Detection and Differentiation of Chlamydiae by Fluorescence In Situ Hybridization
doi: 10.1128/AEM.68.8.4081-4089.2002
Figure Lengend Snippet: Combination of FISH and DFA. The performance of DFA subsequent to FISH allows the identification of chlamydiae (by the rRNA-targeted oligonucleotide probes) simultaneously with the detection of chlamydial antigen (by antichlamydia antibodies). (A) Fluorescent signal derived from DFA using fluorescein-labeled antichlamydia-LPS antibodies. (B) Fluorescent signal derived from FISH with C. pneumoniae-specific probe Cpn-214 labeled with Cy3. (C) Fluorescent signal derived from DAPI staining of chlamydiae and nuclei of the HeLa 229 host cells. (D) Composite image. Bar, 10 μm.
Article Snippet: Chlamydial strains were maintained on cycloheximide-treated
Techniques: Derivative Assay, Labeling, Staining